Darren,
I suspect that what you are hearing are audio artifacts. These are due to using tracks at tempos other than the tempo for which they are designed.
PG Music say that the range of tempo-stretching or tempo-compressing that's workable with Realtracks is -10 bpm and +15 bpm. That is, a RT designed to be played at 100 bpm could be stretched to play around 90 bpm and compressed to play around 115 bpm.
That being said, I find that Piano RTs are particularly sensitive to stretching and compressing. Recently, I had a song that used piano. The tempo was 80 bpm (like your song's tempo) and a RT designed for use at 65 bpm (which fits with the +15 bpm) had too many artifacts for me to use.
You can tell which tempo a RT is designed for by the numerals at the end of its name. For example, RT 887 ends with 100. This indicates that it's recorded at a tempo of 100. This means that it will probably be ok if it's stretch to 90 bpm or compressed to 115 bpm.
RT 1006 in your SEQ file is designed for use at 85 bpm. This should sound reasonably ok at 80 bpm. Because it's being stretched though (i.e. used at a slower tempo), it might have some noticeable audio artifacts. The other RTs you've used are designed for 65, 100, 110 and these are much less likely to work satisfactorily.
As others above have mentioned, MIDI Supertracks are the way to go. They are not affected by tempo in the same way as RTs.
Regards,
Noel