I assume you are using the BIAB feature, the Juke icon. I haven't used this in a long while, since testing. Let's do a few questions to narrow this down, please.

When you look in the Jukebox settings, is Random Order checked? Is Audible Count-in checked? How many seconds is the Delay between songs?

Does this happen on all songs, or only specific ones? If specific ones, when you load the song normally (not in the Jukebox), do you hear the noise?

It would help to tell us if this is Windows or Mac, and what version of BIAB and what Build# you have. I do recall a noise problem in the past involving the Loop setting.
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About your other comments and equipment, the general principle for Gain is to keep the level reasonably as high as you can without any distortion, on each step progressing from the source to the end of the chain. Then you turn things up or down on that last device with a volume knob. It's called gain staging if you would like to read more.

BIAB 2025 Win Audiophile. Software: Studio One 7 Pro, Swam horns, Acoustica-7, Notion 6, Song Master Pro, Win 11 Home. Hardware: Intel i9, 32 Gb; Roland Integra-7, Presonus 192 & Faderport 8, Royer 121, Adam Sub8 & Neumann 120 monitors.